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Image Search Results
Journal: EFSA Journal
Article Title: The European Union summary report on surveillance for the presence of transmissible spongiform encephalopathies ( TSE ) in 2020
doi: 10.2903/j.efsa.2021.6934
Figure Lengend Snippet: Description of the CWD cases in 2020
Article Snippet: , , , , , , , , Screening ,
Techniques: Western Blot
Journal: Journal of Veterinary Medicine
Article Title: A Targeted Survey for Scrapie in Jos Plateau State, Nigeria
doi: 10.1155/2013/841978
Figure Lengend Snippet: Results of clinical examination and laboratory testing of emergency/casualty slaughter goat for scrapie, rabies, and H&E from Jos, Nigeria.
Article Snippet: Caudal brainstem tissues of suspect animals collected postmortem were analyzed for the disease specific form of the prion protein, PrP Sc , using
Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Direct Fluorescent Antibody Test
Journal: Emerging Infectious Diseases
Article Title: Classic Scrapie in Sheep with the ARR/ARR Prion Genotype in Germany and France
doi: 10.3201/eid1308.070077
Figure Lengend Snippet: Biochemical characterization of the prion protein (PrP Sc ) associated with ARR/ARR cases in France and Germany. A) Western blot (stained by monoclonal antibody L42) illustrating that protein kinase (PK)– treated ovine bovine spongiform encephalopathy (BSE) PrP Sc has ≈1-kDa lower molecular mass than PrP Sc from the scrapie cases. Lane 1, S115/04, molecular mass (MM) 20.95 kDa; lane 2, S83, MM 19.96 kDa; lane 3, S95 classic scrapie, MM 19.64 kDa; lane 4, ovine BSE, MM 18.85 kDa. B) PrP Sc PK sensitivity measured by using brain from S83 scrapie case (▲), ARR/ARR BSE in sheep (○), ARQ/ARQ BSE in sheep (●), BSE from bovines (■), an ARR/ARR atypical scrapie case (▼), and 20 randomly selected isolates from sheep with scrapie in France (2 cases shown, represented as □ and ◆). PrP Sc ELISA measurements were performed by using the TeSeE Sheep/Goat rapid test (Bio-Rad) after brain homogenate digestion using a PK concentration ranging from 50 µg/mL to 500 µg/mL. Three tests were performed for each sample and PK concentration.
Article Snippet: For ELISA detection of PrP Sc , commercial TSE rapid tests,
Techniques: Western Blot, Staining, Enzyme-linked Immunosorbent Assay, Concentration Assay
Journal: Emerging Infectious Diseases
Article Title: Classic Scrapie in Sheep with the ARR/ARR Prion Genotype in Germany and France
doi: 10.3201/eid1308.070077
Figure Lengend Snippet: Biochemical properties of prion protein (PrP Sc ) associated with the ARR/ARR scrapie case S83 from France after passage in Tg 338 VRQ mice. A) Western blot mobility of the original S83 ARR/ARR case (lane 3) and S83 passaged in Tg338 (lane 4) were similar and comparable to a classic scrapie isolate (Langlade, lane 2). PrP Sc WB profile of ARR/ARR bovine spongiform encephalopathy (BSE) in sheep (lane 6) and profiles of atypical scrapie case isolates (lane 5) passaged into Tg338 mice were readily distinguishable by their banding pattern or electromobility. B) PrP sc protein kinase (PK) sensitivity of the original S83 isolate (▼) and a classic scrapie isolate (Langlade) (△) compared with S83 (▽) and Langlade (▲) that had been passaged in Tg338 (2 different mice for each isolate). Triplicate ELISA measurements were performed by using the TeSeE Sheep/Goat rapid test (Bio-Rad), after brain homogenate digestion with PK concentration ranging from 50 µg/mL to 500 µg/mL.
Article Snippet: For ELISA detection of PrP Sc , commercial TSE rapid tests,
Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay
Journal: Clinica Chimica Acta; International Journal of Clinical Chemistry
Article Title: Evaluation of 32 rapid tests for detection of antibodies against SARS-CoV-2
doi: 10.1016/j.cca.2021.04.016
Figure Lengend Snippet: Rapid tests included and user-friendliness.
Article Snippet: 7 ,
Techniques: Transferring, Diagnostic Assay